Peptide synthesis by chymotrypsin in frozen solutions
نویسندگان
چکیده
منابع مشابه
Macromolecular synthesis by yeasts under frozen conditions.
Although viable fungi have been recovered from a wide variety of icy environments, their metabolic capabilities under frozen conditions are still largely unknown. We investigated basidiomycetous yeasts isolated from an Antarctic ice core and showed that after freezing at a relatively slow rate (0.8 degrees C min(-1)), the cells are excluded into veins of liquid at the triple junctions of ice cr...
متن کاملFacile synthesis of bis(indolyl)methanes catalyzed by α-chymotrypsin.
A mild and efficient method catalyzed by α-chymotrypsin was developed for the synthesis of bis(indolyl)methanes through a cascade process between indole and aromatic aldehydes. In the ethanol aqueous solution, a green medium, a wide range of aromatic aldehydes could react with indole to afford the desired products with moderate to good yields (from 68% to 95%) using a little α-chymotrypsin as c...
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Ice is selectively intolerant to impurities. A preponderance of implanted anions or cations generates electrical imbalances in ice grown from electrolyte solutions. Since the excess charges are ultimately neutralized via interfacial (H(+)/HO(-)) transport, the acidity of the unfrozen portion can change significantly and permanently. This insufficiently recognized phenomenon should critically af...
متن کاملTransesterification reactions catalyzed by chymotrypsin.
As recently reported, the behavior of acetyl chymotrypsin with ethanol consists in a rapid transfer of the single acetyl group to that alcohol with the formation of ethyl acetate (1). Thus, the splitting of nitrophenyl acetates by chymotrypsin, faster in ethanolic than in aqueous solutions, may be explained by the formation of acetyl chymotrypsin as an intermediate and its subsequent preferenti...
متن کاملInactivation of chymotrypsin by diphenyldiazomethane.
a-Chymotrypsin is a protein composed of three peptide chains linked by disulfide bridges, with amino-terminal half-cystine, isoleucine, and alanine residues.1 The latter two end groups appear upon the enzymic conversion of chymotrypsinogen A (which only contains the amino-terminal half-cystine residue) to a-chymotrypsin. The specificity of a-chymotrypsin as an enzyme (and of other chymotrypsins...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1993
ISSN: 0014-5793
DOI: 10.1016/0014-5793(93)80188-z